keratin 18 k18  (Bioss)

Journal: Frontiers in Immunology

Article Title: A novel oral formulation of the melanocortin-1 receptor agonist PL8177 resolves inflammation in preclinical studies of inflammatory bowel disease and is gut restricted in rats, dogs, and humans

doi: 10.3389/fimmu.2023.1083333

Figure Lengend Snippet: Representative colon immunohistochemistry for K18 sections of DSS colitis-induced rats. Sham (A) is no challenge and no treatment. Placebo/vehicle control (B) is no treatment but DSS challenge. (C) is DSS challenge and treatment with PL8177 (100 µg). (D) is DSS challenge and treatment with mesalazine. (E) Quantification of positive K18 cells in colon crypts versus total crypt cells. Notes: material was limited for sham staining resulting in 2 colon tissues. Bar chart shows mean, SEM, and data points. DSS, dextran sulfate sodium; K18, cytokeratin 18.

Article Snippet: For immunohistochemistry, colon sections were processed for antigen retrieval and immunostained with primary antibody: keratin 18 (K18) (1:400, Bioss, bs-5405R) and with a rabbit-specific biotin-conjugated secondary antibody from HistoWiz (HistoWiz, Inc. Brooklyn, NY).

Techniques: Immunohistochemistry, Control, Staining

Journal: bioRxiv

Article Title: Dissecting the ocular impact of SARS-CoV-2 variants: Analysis of eye globe and retina in animal models

doi: 10.1101/2024.05.29.596469

Figure Lengend Snippet: (A-C) Representative images of K18-hACE2 mice head cross-sections views generated by MRI. The area occupied by the eyes in each plane (sagittal, coronal or orthogonal) is shown in yellow. (D) Eye volume measurements of control (n=10), ancestral- (n=4) and Gamma-infected (n=6) K18-hACE2 mice at 6 dpi. The blue dashed line represents the mean and the black dashed lines represent the S.E.M of non-infected (control) samples.

Article Snippet: Transgenic mice expressing the human hACE2 receptor under the control of the keratin 18 promoter (K18) (B6.Cg-Tg(K18-ACE2)2Prlmn/J) were purchased from Jackson laboratories and bred at ICTB, Fiocruz, Rio de Janeiro, Brazil.

Techniques: Generated, Infection

Journal: bioRxiv

Article Title: Dissecting the ocular impact of SARS-CoV-2 variants: Analysis of eye globe and retina in animal models

doi: 10.1101/2024.05.29.596469

Figure Lengend Snippet: RT-PCR-based quantification of SARS-CoV-2 RNA in the eye globe and in the retina of K18-hACE2-infected mice. Mice were intranasally infected with ancestral (n=4), Gamma (n=3) or Delta (n=3) SARS-CoV-2. The whole eye globe or the retina were harvested at 4dpi. The data indicate means ± S.E.M.

Article Snippet: Transgenic mice expressing the human hACE2 receptor under the control of the keratin 18 promoter (K18) (B6.Cg-Tg(K18-ACE2)2Prlmn/J) were purchased from Jackson laboratories and bred at ICTB, Fiocruz, Rio de Janeiro, Brazil.

Techniques: Reverse Transcription Polymerase Chain Reaction, Infection

Journal: bioRxiv

Article Title: Dissecting the ocular impact of SARS-CoV-2 variants: Analysis of eye globe and retina in animal models

doi: 10.1101/2024.05.29.596469

Figure Lengend Snippet: Mice were intranasally infected with ancestral (n=6), Gamma (n=3) or Delta (n=3) SARS-CoV-2. Eye globes or retina were harvested at 3 and 5 dpi. Relative expression of Il1b and Tnf mRNAs in the eye globes and in the retina of K18-hACE2-infected mice at 4dpi are shown. The data indicate means ± S.E.M.

Article Snippet: Transgenic mice expressing the human hACE2 receptor under the control of the keratin 18 promoter (K18) (B6.Cg-Tg(K18-ACE2)2Prlmn/J) were purchased from Jackson laboratories and bred at ICTB, Fiocruz, Rio de Janeiro, Brazil.

Techniques: Infection, Expressing